DNA Polymerases
Promega GoTaq™ G2 Flexi DNA Polymerase
GoTaq™ G2 Flexi DNA Polymerase is supplied with 5X Green GoTaq™ Flexi Buffer and 5X Colorless GoTaq™ Flexi Buffer and 25mM MgCl2.
Promega M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant
Point mutation of M-MLV RT resulting in no RNase H activity. Suitable for rare or long transcripts.
Promega GoTaq™ G2 DNA Polymerase
GoTaq™ G2 DNA Polymerase is supplied with 5X Green GoTaq™ Reaction Buffer and 5X Colorless GoTaq™ Reaction Buffer.
Promega GoTaq™ Flexi DNA Polymerase
Taq DNA Polymerase. Provided with Green and Colorless Reaction Buffers without MgCl2, and 25mM MgCl2. Green Buffer allows direct gel analysis after amplification. Enhanced performance over standard Taq DNA Polymerase.
Promega M-MLV Reverse Transcriptase
M-MLV RT is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb).
Promega AMV Reverse Transcriptase
AMV RT catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids.
Promega GoTaq MDx DNA Polymerase, 2,500u
GoTaq MDx DNA Polymerase is a full-length form of Taq DNA polymerase that exhibits 5'→3' exonuclease activity.
New England Biolabs, Inc. Q5® High-Fidelity DNA Polymerase – 100 units
Q5 High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (>= 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.
New England Biolabs, Inc. Q5® Hot Start High-Fidelity DNA Polymerase – 500 units
Q5 Hot Start High-Fidelity DNA Polymerase is a high-fidelity, thermostable, hot start DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. The addition of an aptamer-based inhibitor allows room temperature reaction setup. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 Hot Start High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 Hot Start High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (>= 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 Hot Start High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.
G-Biosciences Hot Start Taq DNA Polymerase 2X Master Mix
Hot start Taq DNA Polymerase 2X Master Mix is used for routine PCR, qPCR, genotyping and high GC content template DNA amplification with GC enhancer.
New England Biolabs, Inc. OneTaq Hot Start DNA Polymerase – 1000 units
OneTaq Hot Start DNA Polymerase is an optimized blend of Taq and Deep Vent DNA polymerases combined with an aptamer-based inhibitor. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase and the hot start formulation combines convenience with decreased interference from primer dimers and secondary products. The OneTaq Reaction Buffers and High GC Enhancer have been formulated for robust yields, regardless of a template's GC content. The inhibitor binds reversibly, blocking polymerase activity at temperatures below 45C. The polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature. OneTaq Hot Start DNA Polymerase does not require a separate high temperature incubation step to activate the enzyme and can be used in typical Taq-based cycling protocols. It is supplied with two 5X buffers: (Standard and GC), as well as a High GC Enhancer solution.
New England Biolabs, Inc. Q5® Hot Start High-Fidelity DNA Polymerase – 100 units
Q5 Hot Start High-Fidelity DNA Polymerase is a high-fidelity, thermostable, hot start DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. The addition of an aptamer-based inhibitor allows room temperature reaction setup. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 Hot Start High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 Hot Start High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (>= 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 Hot Start High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes.
Bsu DNA Polymerase Large Fragment, Lyophilized, G-Biosciences
Bsu DNA Polymerase, large fragment is from Bacillus subtilis.
T4 UvsX DNA Recombinase, Glycerol Free, G-Biosciences
T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes.
G-Biosciences Bsu DNA Polymerase, Large Fragment
Bsu DNA Polymerase, Large fragment is used for second strand cDNA synthesis, strand displacement DNA synthesis, random primer labeling and dA-tailing.